Aim: The objective of the study to evaluate the methanolic extracts of aerial parts of Hypericum scabrum L. for in vitro anti-trichomonase activity. Methods: Plant material were collected during the flowering period from natural populations in Sivas province of Turkey. Collected locality is: B6 Sivas: Sivas-Ankara road. The extracts obtained were analyzed by GC-MS to identify possible active substances. Trichomonas vaginalis strain was exposed with plant extracts at concentrations of 5 mg / mL, 2.5 mg / mL, 1.25 mg / mL, 0.6 mg / mL, 0.3 mg / mL, 0.15 mg / mL. Result: The minimum inhibitory concentration (MIC) was found to be 2.5 mg/mL at the end of 2 hours, 1.25 mg/mL at the end of 4 hours, and 0.6 mg/mL at the end of 24 hours after resistant T. vaginalis strains exposed to H. scabrum methanol extract. When susceptible strains are to investigated, at the end of 2 hours the MIC value of 2.5 mg/mL is the lowest dose to the study, at 4 hours later even at the concentration of 0.15 mg/mL, no live parasite is found. Conclusion: The obtained results suggest that the finding can used to further bio-assay guided active compound isolation from this plant as promising resource.
Keywords: Hypericum Scabrum, Anti-Trichomonas Activity, GC-MS